Recombinant Human PTPRM, GST-tagged

• Cat.No.: PTPRM-1861H
• Product Overview: Recombinant humanPTPRM was expressed in anE.coliexpression system using a N-terminal GST tag. MW=56.2kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: PTPRM is a member of the protein tyrosine phosphatase family and can participate in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRM has been shown to mediate cell-cell aggregation through the interaction with another molecule of PTPRM on an adjacent cell. PTPRM can interact with scaffolding protein RACK1/GNB2L1 and this interaction may be necessary for downstream signaling in response to cell-cell adhesion. PTPRM has been shown to be expressed in human pulmonary vascular endothelia where it directly binds to VE-cadherin and regulates both the tyrosine phosphorylation state of VE-cadherin and barrier integrity.
• Purity: > 95% by SDS-PAGE.
• Specific Activity: 680 U/ug. One unit will hydrolyze 1 pmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.
• Application: Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: PTPRM protein tyrosine phosphatase, receptor type, M [ Homo sapiens ]
• Synonyms: PTPRM; protein tyrosine phosphatase, receptor type, M; RPTPM; RPTPU; PTPRL1; hR-PTPu; R-PTP-MU; MGC166994; protein tyrosine phosphatase mu; protein tyrosine phosphatase, receptor type, mu polypeptide; EC 3.1.3.48
• Gene ID: 5797
• mRNA Refseq: NM_001105244
• Protein Refseq: NP_001098714
• MIM: 176888
• UniProt ID: P28827
• Chromosome Location: 18p11.2
• Pathway: Adherens junction; Cell adhesion molecules (CAMs)
• Function: cadherin binding; hydrolase activity; protein binding; receptor activity; transmembrane receptor protein tyrosine phosphatase activity