Recombinant Human RPS6KB1, GST-tagged

• Cat.No.: RPS6KB1-560H
• Product Overview: Recombinant human RPS6KB1 was expressed bybaculovirus in Sf9 insect cellsusing a N-terminal GST tag. MW=74kDa.

Specification

• Cat. No.: RPS6KB1-560H
• Description: P70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).
• Source: Sf9 insect cells using baculovirus.
• Puriry: >60%.
• Specific Activity: 2 pmol/min/μg Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (SER/THR 7, Invitrogen) at room temperature for 1 hour.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Fomulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 [ Homo sapiens ]
• Synonyms: RPS6KB1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70-alpha; p70(S6K)-alpha; p70 S6 kinase, alpha 1; p70 S6 kinase, alpha 2; serine/threonine kinase 14 alpha; EC 2.7.11.1
• Gene ID: 6198
• mRNA Refseq: NM_003161
• Protein Refseq: NP_003152
• MIM: 608938
• UniProt ID: P23443
• Chromosome Location: 17q23.1
• Pathway: Acute myeloid leukemia; ErbB signaling pathway; Fc gamma R-mediated phagocytosis; Insulin signaling pathway; TGF-beta signaling pathway; mTOR signaling pathway; Signaling by Insulin receptor
• Function: ATP binding; nucleotide binding; peptide binding; protein binding; ribosomal protein S6 kinase activity; transferase activity