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Recombinant Bovine Phosphoinositide-3-Kinase, Catalytic, Alpha Polypeptide, GST-tagged

Cat. No.: PIK3CA-1864B
Product Overview: Recombinant BovinePIK3CA was expessed in Sf9 insect cells with a GST-tag.
Description: The PIK3CA andregulatory subunits are co-expressed in Sf9 insect cells. The catalyticsubunit carries a GST-Tag and the heterodimer was purified by affinitychromatography. The GST-Tag facilitates the protein's application in typicalGST pulldown assays. The MW of p110 alpha is 124.3 kDa (without Tag). The MWof p85 alpha is 83.5 kDa.
Source: Sf9 insect cells
Species: Bovine
Form: Liquid supplied in25 mM Tris, pH 8.0 + 50 mM NaCl + 0.5 mM MgCl2 + 50% glycerol.
Concentration: 0.5 mg/ml
Biological Activity: 3 nmol/mg/min usingphosphatidylinositol as the substrate.
Storage: When stored at-20°C, this product is stable for one year. Avoid repeated freeze-thawcycles.
OfficialSymbol: PIK3CA
Gene Name: PIK3CA phosphoinositide-3-kinase,catalytic, alpha polypeptide [ Bos taurus ]
Synonyms: PIK3CA;phosphoinositide-3-kinase, catalytic, alpha polypeptide;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alphaisoform; PI3K; PI3Kalpha; p110alpha; PI3K-alpha; ptdIns-3-kinase p110;PI3-kinase subunit alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinasesubunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine proteinkinase PIK3CA; phosphoinositide-3-kinase catalytic alpha polypeptide;phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunitalpha; EC 2.7.1.153; EC 2.7.11.1; MGC142161; MGC142163; p110-alpha
Gene ID: 282306
mRNA Refseq: NM_174574
Protein Refseq: NP_776999
UniProt ID: P32871
Pathway: Acute myeloidleukemia; Apoptosis; B cell receptor signaling pathway; Chemokine signalingpathway; Colorectal cancer; Endometrial cancer; Fc epsilon RI signalingpathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Inositol phosphatemetabolism; Insulin signaling pathway; Jak-STAT signaling pathway; Leukocytetransendothelial migration; Natural killer cell mediated cytotoxicity;Neurotrophin signaling pathway
Function: 1-phosphatidylinositol-3-kinaseactivity; ATP binding; inositol or phosphatidylinositol kinase activity;insulin receptor substrate binding; nucleotide binding; phosphatidylinositol-4,5-bisphosphate3-kinase activity; phosphotransferase activity, alcohol group as acceptor;transferase activity; protein binding; protein kinase activator activity;protein serine/threonine kinase activity

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