Recombinant Bovine Fibroblast Growth Factor 2 (basic)

• Cat.No.: FGF2-1182B
• Product Overview: Recombinant bovine FGF2is a single, non-glycosylated polypeptide chain containing 172 amino acids.

Specification

• Cat. No.: FGF2-1182B
• Description: FGF2 is asingle-chain polypeptide growth factor that plays a significant role in theprocess of wound healing and is a potent inducer of angiogenesis. Severaldifferent forms of the human protein exist ranging from 18-24 kDa in size dueto the use of alternative start sites within the FGF-2 gene. It has a 55percent amino acid residue identity to FGF-1 and has potent heparin-bindingactivity.
• Source: E. coli
• Species: Bovine
• Form: Lyophilized from asterile filtered solution in PBS, pH 7.4+4% mannitol.
• Molecular Weight: 18.5 kDa
• Purity: > 97% by SDS-PAGEand HPLC analyses
• Endotoxin Level: < 0.1 ng/µg ofFGF2
• Biological Activity: Calculated by thedose dependant proliferation of BALB/c 3T3 cells, the ED50 is <1.0 ng/ml.
• Specific Activity: 1 x 106Units/mg
• Sequences: MTMITNSSSVPGDPLESMAA GSITTLPALP EDGGSGAFPP GHFKDPKRLY CKNGGFFLRI HPDGRVDGVR EKSDPHIKLQLQAEERGVVS IKGVCANRYL AMKEDGRLLA SKCVTDECFF FERLESNNYN TYRSRKYSSW YVALKRTGQYKLGPKTGPGQ KAILFLPMSA KS
• Reconstitution: Centrifuge vialprior to opening. Add steriledistilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL.Further dilutions should be made in appropriate buffered solutions.
• Storage: Stable at 2-8°C, butbest kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeatedfreeze/thaw cycles.
• OfficialSymbol: FGF2

Gene Information

• Gene Name: FGF2 fibroblast growth factor2 (basic) [ Bos taurus ]
• Synonyms: FGF2; fibroblastgrowth factor 2 (basic); heparin-binding growth factor 2; BFGF; HBGF-2; basicfibroblast growth factor
• Gene ID: 281161
• mRNA Refseq: NM_174056
• Protein Refseq: NP_776481
• UniProt ID: P03969
• Chromosome Location: 17
• Pathway: Downstream signalingof activated FGFR; FGFR ligand binding and activation; FGFR1 ligand bindingand activation; FGFR1b ligand binding and activation; FGFR1c ligand bindingand activation; FGFR2 ligand binding and activation; FGFR2c ligand bindingand activation; FGFR3 ligand binding and activation; FGFR4 ligand binding andactivation; FRS2-mediated cascade; IRS-mediated signalling; Insulin receptorsignalling cascade; MAPK signaling pathway; Melanoma
• Function: growth factoractivity; heparin binding; voltage-gated calcium channel activity