Active Native Human GPT

• Cat.No.: GPT-1840H
• Product Overview: Native Human GPT was prepared from Human Liver. CAS# 9000-86-6.

Specification

• Description: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
• Source: Human Liver
• Species: Human
• Form: Lyophilized. Homodimer.
• Bio-activity: >5 U/mg (Dimension® Clinical Chemistry System)
• Molecular Mass: 57,903.6 based on the sequence
• Purity: ≥0.5 mg protein/mg (Coomassie)
• Unit Definition: One unit will catalyze the transamination of one micromole of L-alanine to alpha-ketoglutarate, forming L-glutamate and pyruvate per minute at 37°C and pH 7.8. Measured at 340 nm as one equimolar amount of NAD produced by a coupled reaction.
• Applications: Diagnostic Controls, Calibrators Standards; Clinical Chemistry; Testing/Assay Validation; Life Science; Manufacturing
• Storage: -20°C

Gene Information

• Gene Name: GPT glutamic-pyruvate transaminase (alanine aminotransferase) [ Homo sapiens ]
• Official Symbol: GPT
• Synonyms: GPT; glutamic-pyruvate transaminase (alanine aminotransferase); alanine aminotransferase 1; ALT1; GPT1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; AAT1
• Gene ID: 2875
• mRNA Refseq: NM_005309
• Protein Refseq: NP_005300
• MIM: 138200
• UniProt ID: P24298
• Chromosome Location: 8q24.2-qter
• Pathway: Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem
• Function: 1-aminocyclopropane-1-carboxylate synthase activity; L-alanine:2-oxoglutarate aminotransferase activity; L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding; transaminase activity