Recombinant Human Insulin-Like Growth Factor 1 (somatomedin C)

• Cat.No.: IGF1-606H
• Product Overview: Recombinant humanIGF1 contains 70 amino acids and a 13 amino acid Histidine-based tag. It hasa predicted molecular mass of 9.3 kDa and migrates with an apparent MW 12 kDain SDS-PAGE.

Specification

• Cat. No.: IGF1-606H
• Description: IGF1 is aprotein that in humans is encoded by the IGF1 gene. IGF1 has also beenreferred to as a sulfation factor and its effects were termed nonsuppressibleinsulin-like activity (NSILA) in the 1970s. IGF1 is a hormone similar inmolecular structure to insulin. It plays an important role in childhoodgrowth and continues to have anabolic effects in adults. A synthetic analogof IGF1, mecasermin is used for the treatment of growth failure.
• Source: Hordeum vulgare
• Species: Human
• Molecular Weight: 12 kDa
• Form: Lyophilized from a0.2 sterile filtered solution in 50 mM MES, pH 6.5+100 mM NaCl.
• Purity: > 98% asdetermined by SDS-PAGE analysis
• Endotoxin Level: < 0.005 ng/ug ofIGF1
• Biological Activity: Bioactivity isassayed by measuring the dose dependent effect of human IGF1 on proliferationof FDC-P1 cells, mouse progenitor cells of granulocytes, monocytes andmacrophages. The ED50 for this effect is 10.0 ng/ml.
• Specific Activity: 1 x 105U/mg
• Reconstitution: Centrifuge vialprior to opening. Add steriledistilled water to the vial to fully solubilize the protein to aconcentration not less than 100 ng/ml.
• Storage: Store lyophilizedprotein at -20°C and reconstituted protein in working aliquots at -20°C witha carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage. Pleasenote that the addition of any carrier protein into this product may produceunwanted endotoxin. This depends upon the particular application employed. Avoidrepeated freeze-thaw cycles.
• OfficialSymbol: IGF1

Gene Information

• Gene Name: IGF1 insulin-like growth factor1 (somatomedin C) [ Homo sapiens ]
• Synonyms: IGF1; insulin-likegrowth factor 1 (somatomedin C); IGFI; IGF-I; IGF1A; insulin-likegrowth factor 1; MGF; IGF-IA; IGF-IB; somatomedin-C; OTTHUMP00000195080;OTTHUMP00000195081; OTTHUMP00000195082; OTTHUMP00000195083;OTTHUMP00000195084; mechano growth factor; insulin-like growth factor I;insulin-like growth factor IA; insulin-like growth factor IB
• Gene ID: 3479
• mRNA Refseq: NM_000618
• Protein Refseq: NP_000609
• MIM: 147440
• UniProt ID: P01343
• Chromosome Location: 12q22-q23
• Pathway: Adipogenesis;Aldosterone-regulated sodium reabsorption; Androgen Receptor SignalingPathway; Apoptosis; Diabetes pathways; Dilated cardiomyopathy; EndochondralOssification; Focal Adhesion; Glioma; Hemostasis; Id Signaling Pathway;Long-term depression; Melanoma; MicroRNAs in cardiomyocyte hypertrophy;Oocyte meiosis
• Function: growth factor activity;hormone activity; insulin receptor binding; insulin-like growth factorreceptor binding; integrin binding; protein binding