Recombinant Human Chemokine (C-C motif) Ligand 24

• Cat.No.: CCL24-357H
• Product Overview: Recombinant CCL24 isa disulfide-linked homodimeric protein consisting of 79 amino acid residuesubunits, and migrates as an approximately 9 kDa protein under non-reducing andreducing conditions in SDS-PAGE, and was lyophilzed from 0.2μm filteredconcentrated (1.0mg/ml) solution in 100mM NaCl, 10mM PB, pH 7.0.

Specification

• Cat. No.: CCL24-357H
• Description: Eotaxin belongs tothe platelet factor-4 family of chemokines. The gene symbol is SCYA11. Thefactor is being referred to also as Eotaxin-1 and has been renamed CCL11.Eotaxin induces substantial accumulation of eosinophils in the skin withoutsignificantly affecting the accumulation of neutrophils. Eotaxin is a potentstimulator of both guinea pig and human eosinophils in vitro. The Eotaxinreceptor is a G-protein-coupled receptor selectively expressed in humaneosinophils.
• Source: Human
• Host: E. coli
• Purity: >97%, asdetermined by SDS-PAGE and HPLC.
• Sequence: MAGLMTIVTSLLFLGVCAHH IIPTGSVVIP SPCCMFFVSK RIPENRVVSY QLSSRSTCLK AGVIFTTKKG QQFCGDPKQEWVQRYMKNLD AKQKKASPRA RAVAVKGPVQ RYPGNQTTC
• Molecular Weight: Native human CCL24has a calculated molecular mass of approximately 9 kDa.
• Biological Activity: Activity wasdetermined by the ability to induce chemotaxis of purified eosinophils atconcentrations ranging between 50-100 ng/ml.
• Endotoxin: Endotoxin contentwas assayed using a LAL gel clot method. Endotoxin level was found to be lessthan 0.1 ng/µg (1EU/µg).
• Reconstitution: A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Storage: The lyophilizedprotein is stable for at least 2 years from date of receipt at -20° C. Uponreconstitution, this cytokine can be stored in working aliquots at 2° - 8° Cfor one month, or at -20° C for six months, with a carrier protein withoutdetectable loss of activity. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: CCL24
• Pathways: Chemokine signalingpathway; Cytokine-cytokine receptor interaction

Gene Information

• Gene Name: CCL24chemokine (C-C motif) ligand 24 [ Homo sapiens ]
• Synonyms: CCL24;chemokine (C-C motif) ligand 24; Ckb-6; MPIF2; MPIF-2; SCYA24; C-C motifchemokine 24; CK-beta-6; eotaxin-2; OTTHUMP00000210224; OTTHUMP00000210225;small-inducible cytokine A24; eosinophil chemotactic protein 2; myeloidprogenitor inhibitory factor 2; small inducible cytokine subfamily A(Cys-Cys), member 24
• Gene ID: 6369
• mRNA Refseq: NM_002991
• Protein Refseq: NP_002982
• MIM: 602495
• UniProt ID: O00175
• Chromosome Location: 7q11.23
• Function: chemokine activity