Recombinant Human Charged Multivesicular Body Protein 4A, His-tagged

• Cat.No.: CHMP4A-1376H
• Product Overview: Recombinant humanCHMP4A protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CHMP4A(Chromatin-modifying protein 4a) belongs to the SNF7 family and functions aschromatin-modifying proteins. It probable core component of the endosomalsorting required for transport complex III (ESCRT-III) which is involved inmultivesicular bodies (MVBs) formation and sorting of endosomal cargoproteins into MVBs. Also during HIV-1 infection, the virus uses the ESCRT-IIIcomplex to mediate budding and exocytosis of viral proteins via theassociation of CHMP4 and a protein recruited by HIV-1 p6, which is present inviral Gag assembly and budding. CHMP4A expressed at higher level in heart,kidney, liver and skeletal muscle.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200mM NaCl, 0.1mMPMSF, 1mM EDTA.
• Molecular Weight: 32.0 kDa (285aa),confirmed by MALDI-TOF.
• Purity: > 90% by SDS-PAGE
• Concentration: 0.25 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRLFGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLAQTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAISRPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQLAEWVS
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: ESCRT-III complex;Endocytosis; Endosomal Sorting Complex Required For Transport (ESCRT);Membrane Trafficking

Gene Information

• Gene Name: CHMP4A charged multivesicularbody protein 4A [ Homo sapiens ]
• Official Symbol: CHMP4A
• Synonyms: CHMP4A; charged multivesicular body protein 4A; SNF7;CHMP4; SHAX2; CHMP4B; SNF7-1; VPS32A; HSPC134; VPS32-1; FLJ61658; C14orf123;MGC142093; MGC142095; charged multivesicular body protein 4a;OTTHUMP00000164694; OTTHUMP00000235236; chromatin modifying protein 4A; SNF7homolog associated with Alix-2; Snf7 homologue associated with Alix 2;vacuolar protein sorting-associated protein 32-1
• Gene ID: 29082
• mRNA Refseq: NM_014169
• Protein Refseq: NP_054888
• MIM: 610051
• UniProt ID: Q9BY43
• Chromosome Location: 14q11.2
• Function: lipid binding