|Cat. No. :
|Product Overview :
||Recombinant human LDLRAP1 protein, fused to His-tag atN-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||LDLRAP1(Low density lipoprotein receptor adapter protein 1) is a cytosolic proteinwhich contains a phosphotyrosine binding (PTD) domain. LDLRAP1 is a cytosolic adaptor that couples LDLR to endocyticmachinery. Defects in LDLRAP1are the cause of autosomal recessive hypercholesterolemia (ARH). ARH is adisorder caused by defective internalization of LDL receptors (LDLR) in theliver.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl.
|Molecular Weight :
||36.1 kDa (328aa), confirmed by MALDI-TOF.
||> 90%by SDS-PAGE
||0.5mg/ml(determined by Bradford assay)
|Sequences of aminoacids :
||MGSSHHHHHHSSGLVPRGSH MDALKSAGRA LIRSPSLAKQ SWGGGGRHRK LPENWTDTRE TLLEGMLFSLKYLGMTLVEQ PKGEELSAAA IKRIVATAKA SGKKLQKVTL KVSPRGIILT DNLTNQLIEN VSIYRISYCTADKMHDKVFA YIAQSQHNQS LECHAFLCTK RKMAQAVTLT VAQAFKVAFE FWQVSKEEKE KRDKASQEGGDVLGARQDCT PPLKSLVATG NLLDLEETAK APLSTVSANT TNMDEVPRPQ ALSGSSVVWE LDDGLDEAFSRLAQSRTNPQ VLDTGLTAQD MHYAQCLSPV DWDKPDSSGT EQDDLFSF
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
|Pathways||Chylomicron-mediatedlipid transport; Endocytosis; LDL-mediated lipid transport; Lipid digestion,mobilization, and transport ;Lipoprotein metabolism; Metabolism of lipids andlipoproteins|