Recombinant Porcine Tumor Necrosis Factor

Cat. No. : TNF-5525P
Product Overview : Recombinant porcineTNF is produced in E. coli and purified via sequential chromatography.
Description : Tumor necrosisfactor (TNF, cachexin or cachectin formerly known as tumor necrosisfactor-alpha or TNF-α) is a cytokine involved in systemic inflammation and isa member of a group of cytokines that stimulate the acute phase reaction. Itis produced chiefly by activated macrophages, although it can be produced by othercell types as well. The primary role of TNF is in the regulation of immunecells. TNF, being an endogenous pyrogen, is able to induce fever, to induceapoptotic cell death, to induce sepsis (through IL1 & IL6 production), toinduce cachexia, induce inflammation, and to inhibit tumorigenesis and viralreplication.
Source : E. coli
Species : Porcine
Form : Lyophilized, carrierfree
Molecular Weight : 17.1 kDa
Purity : >95% asdetermined by SDS-PAGE analysis
Endotoxin : <0.1 ng/µg
Biological Activity : ED50 range =0.001-0.015 ng/mL, determined by the dose dependent cytotoxicity of porcinePK15 cells. Optimal concentration for individual application should bedetermined by a dose response assay.
Reconstitution : We recommend thatthe vial be briefly centrifuged prior to opening to bring the contents to thebottom. Lyophilized porcine TNF-alpha should be reconstituted in sterile,deionized H2O to 0.1-1.0 mg/mL to regain full activity. These stock solutionsshould be apportioned into working aliquots and stored below -20°C. Furtherdilutions should be made in low endotoxin medium or buffered solution withheat inactivated FCS or tissue culture grade BSA.
Storage : Lyophilized porcineTNF-alpha should be stored at 2-8°C, preferably desiccated. Store reconstitutedporcine TNF-alpha below -20°C (not in a frost-free freezer). Keep freeze-thawcycles to a minimum.
OfficialSymbol : TNF
Gene Name : TNF tumor necrosis factor [ Susscrofa ]
Synonyms : TNF; tumor necrosisfactor; TNFa; TNF-a; TNF-alpha; tumor-necrosis factor; cachectin; tumornecrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2);tumor necrosis factor ligand superfamily member 2
Gene ID : 397086
mRNA Refseq : NM_214022
Protein Refseq : NP_999187
UniProt ID : P23563
Chromosome Location : 7p11-q11; 7
Pathway : Adipocytokinesignaling pathway; Cytokine-cytokine receptor interaction; Death ReceptorSignalling; Extrinsic Pathway for Apoptosis; Fc epsilon RI signaling pathway;Graft-versus-host disease; Hematopoietic cell lineage; Leishmaniasis; MAPKsignaling pathway; Natural killer cell mediated cytotoxicity; Osteoclastdifferentiation; RIG-I-like receptor signaling pathway; Systemic lupuserythematosus; TGF-beta signaling pathway; Type II diabetes mellitus

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