||Recombinant Rhesus macaque CSF3 was expressed in E.coli.
||Colony Stimulating Factor 3 (CSF3), previously known as granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine and mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Many cell types secreted this protein after LPS, IL-1 or TNFα activation, including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express CSF3 constitutively. CSF3 is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition it may function in some adhesion or recognition events at the cell surface.
||Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.
||Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0×10^7 IU/mg.
||~18.9 kDa (174 amino acids)
||>98% by SDS-PAGE and HPLC analyses.
||Storage/Handling: Store desiccated at -20ºC.