||Recombinanthuman full length MAPK9, His-tagged. Activated by coexpression with untaggedMAP2K7 and subsequently purified using Ni-NTA chromatography followed by ionexchange chromatography.
||MAPK9is an enzyme that in humans is encoded by the MAPK9 gene.The protein encodedby this gene is a member of the MAP kinase family. MAP kinases act as anintegration point for multiple biochemical signals, and are involved in awide variety of cellular processes such as proliferation, differentiation,transcription regulation and development.
||Liquid in50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
||≥ 90% as determinedby SDS-PAGE analysis
||93 nmoleof phosphate transferred to ATF2 substrate per minute per mg of total proteinat 30°C. Activity determined at a final protein concentration of 3.33 μg/ml.
||Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
|Function||ATP binding; JUN kinaseactivity; MAP kinase activity; nucleotide binding; protein binding; proteinserine/threonine kinase activity; transferase activity|