||Therecombinant human PRKCA was expressed in E. coli and purified by Ni-agarose chromatography.It is suitable for labeling PKC substrates.
||PRKCA isa serine/threonine kinase and a member of the conventional (classical) PKCs(cPKCs), which have four conserved (C1 to C4) regions. This ubiquitouslyexpressed PKC isotype is activated in response to many different kinds ofstimuli and translocates from cytosol to the specialized cellularcompartments (nucleus, focal adhesion, caveolae, etc.) where it is presumedto work. Therefore, PRKCA has been implicated in a variety of cellularfunctions including proliferation, apoptosis, differentiation, motility, andinflammation.
||Liquid ina 20 mM NaH2PO4 buffer, pH 7.8, + 500 mM NaCl + 1 mM Na-orthovanadate + 5 mMNaF + 40 mM β-glycerophosphate + 5% glycerol.
||> 95% by SDS-PAGE
||>200,000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to PKCpeptide QKRPSQRSKYL per min at 30°C).
||< 0.1ng/μg of protein.
||For longterm storage, aliquot and freeze at -80°C. Stable for 1 year. Avoid repeated freeze-thawcycles.
|Function||nucleotide binding; proteinbinding; protein kinase activity; protein serine/threonine kinase activity; zincion binding; metal ion binding; histone kinase activity (H3-T6 specific); enzymebinding; ATP binding|