||Recombinanthuman full length PRKCB with N-terminal GST tag was expressed in Sf9 insectcells.
||PRKCB isa member of the protein kinase C (PKC) family of serine- andthreonine-specific protein kinases that can be activated by calcium andsecond messenger diacylglycerol and phosphorylate a wide variety of proteintargets known to be involved in diverse cellular signaling pathways. PRKCBhas been reported to be involved in many different cellular functions such asB-cell activation, apoptosis induction, endothelial cell proliferation, andintestinal sugar absorption.
||Sf9 insect cells
||Liquid in50 mM Tris-HCl, pH 7.5 + 150 mM NaCl + 0.25 mM DTT + 0.1 mM EGTA + 0.1 mMEDTA + 0.1 mM PMSF + 25% glycerol.
||~ 150 kDa
||> 90% asdetermined by densitometry
||Stablefor 1 year in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
|Function||nucleotide binding; proteinbinding; protein kinase activity; protein serine/threonine kinase activity; zincion binding; metal ion binding; histone kinase activity (H3-T6 specific); enzymebinding; ATP binding|