Active Recombinant Human KIT Protein, His-tagged

• Cat.No.: KIT-389H
• Product Overview: Recombinant Human Kit protein (a.a. 544-976) was expressed in a Baculovirus infected Sf9 cell expression system with N-terminal His-tag.

Specification

• Description: This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: 16 U/mg
• Molecular Mass: 53.4 kDa,
• Purity: ≥53%
• Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30 centigrade.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Concentration: 0.09 mg/ml
• Storage buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole.

Gene Information

• Gene Name: KIT KIT proto-oncogene receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: KIT
• Synonyms: PBT; SCFR; C-Kit; CD117
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213.1
• MIM: 164920
• UniProt ID: P10721