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Recombinant Human Pim-1 Oncogene, His-tagged

Cat. No.: PIM1-1288H
Product Overview: Recombinant humanfull length PIM1, His-tagged. The proto-oncogene PIM1 is upregulated inprostate cancer. No special measures were taken to activate this kinase.
Description: PIM1 is aproto-oncogene which encodes for the serine/threonine kinase of the samename. The PIM1 oncogene was first described in relation to murine T-celllymphomas, as it was the locus most frequently activated by the Moloney murineleukemia virus. Subsequently, the oncogene has been implicated in multiplehuman cancers, including prostate cancer, acute myeloid leukemia and otherhematopoietic malignancies.
Source: Insect cells
Species: Human
Form: Liquid in 50 mMTris, pH 7.5 + 500 mM NaCl + 0.5 mM EDTA + 0.05% Triton X-100 + 2 mM DTT +50% glycerol.
Concentration: 0.18 mg/mL totalprotein as measured using the Bradford protein assay with BSA as a standard.
Molecular Weight: 40.7 kDa
Purity: 90% as determined bya Coomassie blue-stained SDS-PAGE gel
Mass Spectrometry: The protein wasdigested with trypsin followed by LC-MS/MS. The resulting MS/MS data verifiedPIM1 identity from 8 peptides that covered 13% of the amino acid sequence ofthe recombinant protein.
Assay Conditions: The enzyme waspre-diluted in enzyme dilution buffer and assayed in 50 mM Tris (pH 7.5), 10mM MgCl2, 2 mM DTT, 0.025% Triton® X-100, 200 µM ATP, 100 µM PIM1 peptidesubstrate and trace [32P]-γ-ATP for 10 minutes at 30°C.
Specific Activity: 8680 nmole ofphosphate transferred to PIM1 peptide substrate (AKKRRLSA) per minute per mgof total protein at 30°C. Activity determined at a final proteinconcentration of 0.083 µg/mL.
Dilution Buffer: 20 mM Tris (pH 7.5),0.05% Triton® X-100, 0.1 mg/mL BSA, 0.5 mM Na3VO4, 2 mM DTT, 10% Glycerol.
Storage Buffer: 50 mM Tris (pH 7.5),500 mM NaCl, 0.5 mM EDTA, 0.05% Triton® X-100, 2 mM DTT, 50% Glycerol.
Storage: Store at -80°C. Atfirst use, aliquot and store at -80°C to avoid multiple freeze-thaws. Ifproperly stored at -80°C, this product is stable for 6 months from date ofpurchase. This protein comes out of solution at low salt concentrations.Avoid repeated freeze-thaw cycles.
OfficialSymbol: PIM1
Gene Name: PIM1 pim-1 oncogene [ Homo sapiens ]
Synonyms: PIM1; pim-1oncogene; PIM; Proto-oncogene serine/threonine-protein kinase Pim-1; EC2.7.11.1
Gene ID: 5292
mRNA Refseq: NM_002648
Protein Refseq: NP_002639
MIM: 164960
UniProt ID: P11309
Chromosome Location: 6p21.2
Pathway: Acute myeloidleukemia; Jak-STAT signaling pathway; C-MYB transcription factor network;IL-5 Signaling Pathway; Role of Calcineurin-dependent NFAT signaling inlymphocytes; Validated targets of C-MYC transcriptional activation
Function: ATP binding;nucleotide binding; manganese ion binding; metal ion binding; proteinbinding; protein serine/threonine kinase activity; transcription factorbinding

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