||Recombinant human CTDSPLprotein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||CTDSPL,also known as CTD small phosphatase-like protein, preferentially catalyzesthe dephosphorylation of Ser-5 within the tandem 7 residues repeats in theC-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT.
||23.9 kDa(209aa) confirmed by MALDI-TOF
||> 90%by SDS-PAGE
||0.5mg/ml (determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMKYLLP EVTVLDYGKK CVVIDLDETL VHSSFKPISN ADFIVPVEID GTIHQVYVLKRPHVDEFLQR MGQLFECVLF TASLAKYADP VADLLDRWGV FRARLFRESC VFHRGNYVKD LSRLGRELSKVIIVDNSPAS YIFHPENAVP VQSWFDDMTD TELLDLIPFF EGLSREDDVY SMLHRLCNR
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||BMPreceptor signaling; Regulation of cytoplasmic and nuclear SMAD2/3 signaling