Recombinant Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) Small Phosphatase-Like, His-tagged

• Cat.No.: CTDSPL-1922H
• Product Overview: Recombinant human CTDSPLprotein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: CTDSPL-1922H
• Description: CTDSPL,also known as CTD small phosphatase-like protein, preferentially catalyzesthe dephosphorylation of Ser-5 within the tandem 7 residues repeats in theC-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A.
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT.
• Molecular Weight: 23.9 kDa(209aa) confirmed by MALDI-TOF
• Purity: > 90%by SDS-PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMKYLLP EVTVLDYGKK CVVIDLDETL VHSSFKPISN ADFIVPVEID GTIHQVYVLKRPHVDEFLQR MGQLFECVLF TASLAKYADP VADLLDRWGV FRARLFRESC VFHRGNYVKD LSRLGRELSKVIIVDNSPAS YIFHPENAVP VQSWFDDMTD TELLDLIPFF EGLSREDDVY SMLHRLCNR
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: BMPreceptor signaling; Regulation of cytoplasmic and nuclear SMAD2/3 signaling

Gene Information

• Gene Name: CTDSPL CTD (carboxy-terminaldomain, RNA polymerase II, polypeptide A) small phosphatase-like [ Homosapiens ]
• Official Symbol: CTDSPL
• Synonyms: CTDSPL; CTD (carboxy-terminal domain, RNApolymerase II, polypeptide A) small phosphatase-like; PSR1; SCP3; HYA22;RBSP3; C3orf8; CTD small phosphatase-like protein; CTDSP-like;NIF-like protein; small CTD phosphatase 3; NLI-interacting factor 1; smallC-terminal domain phosphatase 3; nuclear LIM interactor-interacting factor 1;RB protein serine phosphatase from chromosome 3; carboxy-terminal domain RNApolymerase II polypeptide A small phosphatase 3; EC 3.1.3.16; chromosome3 open reading frame 8
• Gene ID: 10217
• mRNA Refseq: NM_001891
• Protein Refseq: NP_001882
• MIM: 608592
• UniProt ID: O15194
• Chromosome Location: 3p21.3
• Function: hydrolase activity; metal ion binding;molecular function; phosphoprotein phosphatase activity