||Recombinant, human SENP1 Catalytic Domain consistingof amino acids 415-643 was fused with a His Tag.
||The covalent modification of proteins by SUMO is reversibleand is mediated by SENP enzymes. SENPs cleave residues from the C-terminus ofSUMO precursor proteins to generate the mature and active form which containsthe conserved C-terminal di-glycine. These enzymes also reverse the processof SUMOylation by removing poly-SUMO conjugates from substrate proteins.SENP1 is active against SUMO-1, SUMO-2 and SUMO-3 in vitro but not ubiquitinor NEDD8.
||In 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT.
||> 95% bySDS-PAGE
||Typical enzymeconcentration to support hydrolysis of substrates in vitro is 50-500 nM depending onconditions. Pre-incubation (15 min) with 10 mM DTT isrecommended toachieve maximum activity
||Store at-80°C. Avoid multiple freeze/thaw cycles.