Active Recombinant Mouse Integrin Alpha 4, His-tagged

• Cat.No.: Itga4-5665M
• Product Overview: RecombinantMouse Itga4 protein was expressed in Chinese HamsterOvary cell line. Mouse Integrin α4, Tyr34-Gln970 and Mouse Integrin β1Gln21-Asp728 with an N-terminal and with a C-terminal 6-His tag.

Specification

• Cat. No.: Itga4-5665M
• Description: Itga4 isan integrin family adhesion receptor that shares the β1 subunit with elevenother family members and the α4 subunit with Integrin α4β7. The non-covalent heterodimerof 150 kDa α4/CD49d and 130 kDa β1/CD29 type I transmembrane glycoprotein subunitsmediates cell adhesion to VCAM-1/CD106 on other cells and the CS-1 fragmentof fibronectin in the extracellular matrix. The α4 extracellular domain (ECD)contains an N-terminal β-propeller structure, followed by domains termedthigh, calf-1 and calf-2.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• N-terminalSequence: Tyr34 (Integrin α4)& Gln21 predicted (Integrin β1)
• MolecularWeight: 112.9 kDa (Integrinα4) & 86.4 kDa (Integrin β1)
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of VCAM-1transfected Chinese hamster ovary (CHO) cells. When 5x104cells/well are added to Recombinant Mouse Itga4 coatedplates (10μg/mL, 100μL/well), approximately 55-85% will adhere after 1 hourat 37°C.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 145-160kDa and 120-140 kDa, reducing donditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Itga4integrin alpha 4 [ Mus musculus ]
• Official Symbol: Itga4
• Synonyms: Itga4; integrinalpha 4; CD49D; integrin alpha-4; integrinalpha-IV; LPAM subunit alpha; VLA-4 subunit alpha; VLA-4 receptor, alpha 4subunit; CD49 antigen-like family member D; lymphocyte Peyer patch adhesionmolecules subunit alpha
• Gene ID: 16401
• mRNA Refseq: NM_010576
• Protein Refseq: NP_034706
• UniProt ID: P09055
• Chromosome Location: 2
• Pathway: Adaptive ImmuneSystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC); Cell adhesionmolecules (CAMs); Cell surface interactions at the vascular wall; Dilatedcardiomyopathy; ECM-receptor interaction; Focal Adhesion; Hemartopoietic celllineage; Hemostasis; Hypertrophic cardiomyopathy (HCM); Immune System;Integrin cell surface interactions; Integrin-mediated cell adhesion;Intestinal immune network for IgA production; Leishmaniasis; Leukocytetransendothelial migration; Regulation of actin cytoskeleton; SignalTransduction
• Function: fibronectin binding;fibronectin binding