Active Recombinant Mouse Amine Oxidase, Copper Containing 3, His-tagged

• Cat.No.: Aoc3-561M
• Product Overview: Recombinant Mouse Aoc3protein was expressed in Spodoptera frugiperda, Sf 21 (baculovirus)-derived withan N-terminal Met, 10-His tag, and aGly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Ile-Glu-Gly-Arg linker. Gly27-Asn765.

Specification

• Cat. No.: Aoc3-561M
• Description: Aoc3 is a copperamine oxidase with a topaquinone cofactor. Aoc3 is a type II integralmembrane protein, but a soluble form of the enzyme is present in human serum,and its level increases in diabetes and some inflammatory liver diseases.Aoc3 catalyzes the oxidative deamination of small primary amines such asmethylamine, benzylamine, and aminoacetone in a reaction that produces analdehyde, ammonia, and H2O2. The enzyme is sensitive toinhibition by semicarbazide. Aoc3 expression is highest in the endothelium oflung, heart, and intestine, but low in tissues such as brain, spleen, kidney,and liver.
• Source: Spodopterafrugiperda, Sf 21 (baculovirus)-derived.
• Form: Supplied as a 0.2 μmfiltered solution in Tris and NaCl.
• N-terminalSequence: Met
• Molecular Weight: 85 kDa
• Activity: Measured by itsability to produce hydrogen peroxide during the oxidation of benzylamine. Thespecific activity is > 10 pmoles/min/μg, as measured under the described conditions.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 80-90kDa, reducing conditions.
• Purity: > 95%, by SDSPAGEunder reducing conditions and visualized by Colloidal Coomassie® Blue stainat 5 μg per lane.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-70°C as supplied. 3 months, -70°C under sterile conditions after opening.

Gene Information

• Gene Name: Aoc3 amine oxidase, coppercontaining 3 [ Mus musculus ]
• Official Symbol: Aoc3
• Synonyms: Aoc3; amine oxidase,copper containing 3; SSAO; VAP1; membrane primary amine oxidase; VAP-1;copper amine oxidase; vascular adhesion protein 1; semicarbazide-sensitiveamine oxidase; EC 1.4.3.21
• Gene ID: 11754
• mRNA Refseq: NM_009675
• Protein Refseq: NP_033805
• Chromosome Location: 11; 11 B2-B5
• Pathway: Glycine; Metabolicpathways; Phenylalanine metabolism; Tyrosine metabolism; beta-Alaninemetabolism; beta-alanine biosynthesis I; threonine degradation III (tomethylglyoxal)
• Function: aliphatic-amineoxidase activity; aminoacetone:oxygen oxidoreductase(deaminating) activity;calcium ion binding; cation channel activity; copper ion binding; metal ionbinding; oxidoreductase activity; phenethylamine:oxygen oxidoreductase(deaminating) activity; primary amine oxidase activity; proteinhomodimerization activity; quinone binding; tryptamine:oxygen oxidoreductase(deaminating) activity