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Recombinant Mouse Arylsulfatase G, His-tagged

Cat. No.: Arsg-574M
Product Overview: Recombinant Mouse Arsgprotein was expressed in Chinese Hamster Ovary cell line with a C-terminal 10-Histag. Gly17-Val525.
Description: Arsg is a member ofthe aryl sulfatase family of enzymes with a high degree of amino acidsequence similarity to ARSA and ARSB. Arsg mRNA is expressed in a ubiquitousmanner, being detected in most adult and embryonic tissues of the mouse.
Source: Chinese Hamster Ovarycell line, CHO-derived.
Form: Supplied as a 0.2 μmfiltered solution in Tris and NaCl.
N-terminalSequence: Gly17
Molecular Weight: 57 kDa
Activity: Measured by itsability to hydrolyze the substrate 4Nitrocatechol Sulfate (PNCS). Thespecific activity is > 1,500 pmoles/min/μg, as measured under thedescribed conditions.
Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
SDS-PAGE: 65 kDa,reducing conditions.
Purity: > 95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Use a manual defrostfreezer and avoid repeated freezethaw cycles. 6 months from date of receipt, -20to -70°C as supplied. 3 months, -20 to -70°C under sterile conditions afteropening.
Gene Name: Arsg arylsulfatase G [ Musmusculus ]
Official Symbol: Arsg
Synonyms: Arsg; arylsulfataseG; AI846872;6330406P08Rik; arylsulfatase G; ASG
Gene ID: 74008
mRNA Refseq: NM_001166177
Protein Refseq: NP_001159649
Chromosome Location: 11 E1; 11
Pathway: Lysosome
Function: arylsulfataseactivity; catalytic activity; hydrolase activity; metal ion binding; sulfuricester hydrolase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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