"Fzd8" Related Products

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Recombinant Mouse Frizzled Homolog 8 (Drosophila), Fc-tagged

Cat. No.: Fzd8-593M
Product Overview: RecombinantMouse Fzd8 protein was expressed in Murine myeloma cell line with the Fc partof Human IgG1 Pro100-Lys330 (C-terminus) and Mouse Frizzled-8 (Ala25-Pro173)&(Ala28-Pro173)(N-terminus).
Description: Fzd8 is one of atleast ten seventransmembrane (7TM) glycoprotein of the Frizzled family of Wntreceptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement,with low density lipoprotein receptor related proteins LRP-5 or LRP-6 actingas co-receptors stabilizes β-catenin and promotes gene transcription that isimportant in development and tissue maintenance.
Source: Murine myeloma cellline, NS0-derived
Form: Supplied as a 0.2 μmfiltered solution in PBS.
N-terminal Sequence: Ala25 & Ala28
Molecular Weight: 43 kDa
Activity: Measured by itsability to interfere with the axis-inducing activity of Xwnt-8 mRNA in early Xenopusembryos. In a population of embryos, addition of 1 ng of mouse Frizzled8/ FcChimera protein per embryo resulted in 50-100% reduction in the number ofsecondary axes produced by 10-20 pg of Xwnt-8 mRNA. Also measured by itsability to bind biotinylated rmWnt-5a in a functional ELISA with an estimatedKD < 1 nM.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
SDS-PAGE: 59-60 kDa, reducingconditions
Purity: >95%, by SDS-PAGEunder reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 250μg/mL in sterile PBS.
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to-70 °C under sterile conditions after reconstitution.
Gene Name: Fzd8 frizzled homolog 8(Drosophila) [ Mus musculus ]
Official Symbol: Fzd8
Synonyms: Fzd8;frizzled homolog 8 (Drosophila); Fz8; frizzled-8; Fz-8; mFz8
Gene ID: 14370
mRNA Refseq: NM_008058
Protein Refseq: NP_032084
UniProt ID: Q61091
Chromosome Location: 18
Pathway: Basal cellcarcinoma; Class B/2 (Secretin family receptors); GPCR ligand binding; HTLV-Iinfection; Melanogenesis; Pathways in cancer; Signal Transduction; Signalingby GPCR; Wnt Signaling Pathway; Wnt signaling network
Function: G-proteincoupled receptor activity; PDZ domain binding; Wnt-activated receptoractivity; Wnt-protein binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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