Active Recombinant Human Glucosaminyl (N-acetyl) Transferase 1, Core 2, His-tagged

• Cat.No.: GCNT1-617H
• Product Overview: RecombinantHuman GCNT1 protein was expressed in Murine myeloma cellline. Arg33-His428,with an N-terminal CD33 signals sequence and a C-terminal 6-His tag.

Specification

• Cat. No.: GCNT1-617H
• Description: MucintypeO-glycans are initiated with an O-GalNAc attachment to a serine or threoninein a polypeptide. The O-GalNAc residues are subsequently extended by variousglycosyltransferases resulting in different types of O-glycans. Most O-glycanscontain the core 1 structure, Galβ-13GalNAc. GCNT1 convertsthe core 1 O-glycan to core 2 O-glycan, Galβ1-3 (GlcNAcβ1-6) GalNAc, via theaddition of a GlcNAc residue. Various ligand carbohydrates can be formed fromcore 2 branched oligosaccharides.
• Source: Mammalian cells
• Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and Brij35.
• N-terminalSequence: Arg33
• MolecularWeight: 47 kDa
• Activity: Measuredby its ability to transfer GlcNAc from UDP-GlcNAc to bovine asialofetuin. Thespecific activity is > 35 pmol/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 50-57kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by Colloidal CoomassieBlue stain at 5 μg per lane.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.

Gene Information

• Gene Name: GCNT1 glucosaminyl (N-acetyl)transferase 1, core 2 [ Homo sapiens ]
• Official Symbol: GCNT1
• Synonyms: GCNT1; glucosaminyl(N-acetyl) transferase 1, core 2; glucosaminyl (N acetyl) transferase 1, core2 (beta 1, 6 N acetylglucosaminyltransferase); NACGT2; beta-1,3-galactosyl-O-glycosyl-glycoprotein; beta-1, 6-N-acetylglucosaminyltransferase
• Gene ID: 2650
• mRNA Refseq: NM_001097633
• Protein Refseq: NP_001091102
• MIM: 600391
• UniProt ID: Q02742
• Chromosome Location: 9q13
• Pathway: Metabolic pathways;Metabolism of proteins; Mucin type O-Glycan biosynthesis; O-glycanbiosynthesis; O-linked glycosylation of mucins; Post-translational proteinmodification
• Function: beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase activity; transferase activity, transferringglycosyl groups