| Cat. No. : |
GPC6-5629H |
| Description : |
The glypicans(glypiated proteoglycans) are a small multigene family of GPI-linkedproteoglycans that likely play a key role in embryonic morphogenesis. Thereare six known mammalian glypicans. They all share a common-sized protein coreof 60-70 kDa, an N-terminus which likely forms a compact globular domain, 14conserved cysteines that form multiple intrachain disulfide bonds, and anumber of C-terminal N-and O-linked carbohydrate attachment sites. Based onexon organization and the location of O-linked glycosylation sites, at leasttwo subfamilies of glypicans are known, with one subfamily containingglypicans-1, 2, 4, and 6, and another subfamily containing glypicans-3 and 5. |
| Source : |
Mammalian cells |
| Form : |
Lyophilized from a0.2 um filtered solution in PBS. |
| N-terminalSequence : |
Asp24 & Ser355 |
| MolecularWeight : |
38 kDa and 20 kDa |
| Activity : |
Measuredby its binding ability in a functional ELISA. When biotinylated rmWnt-3a (0.5μg/mL, 100 μL/well) is captured on streptavidin-coated plate, rhGlypican-6binds with an apparent KD < 10 nM. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
38 kDaand 27-29 kDa, reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 100μg/mL in PBS. |
| Storage : |
Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution. |
