"Fgf8" Related Products

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Recombinant Mouse Fibroblast Growth Factor 8

Cat. No.: Fgf8-539M
Product Overview: Recombinant Mouse Fgf8protein was expressed in E. coli with an N-terminal Met. Gln23-Arg268(Ala35Pro).
Description: Fgf8 is a member ofthe fibroblast growth factor family that was originally discovered as agrowth factor essential for the androgen-dependent growth of mouse mammarycarcinoma cells. Alternate splicing of mouse Fgf8 mRNA generates eightsecreted isoforms, designated a-h, but only Fgf8a, b, e and f exist in human.Fgf8 contains a 22 amino acid (aa) signal sequence, an N-terminal domain thatvaries according to the isoform (84 aa for Fgf8c; 20 aa for the shortest,Fgf8a), a 125 aa FGF domain and a 37 aa prolinerich C-terminal sequence.
Source: E. coli-derived
Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
N-terminalSequence: Met
Molecular Weight: 28 kDa
Activity: Measuredin a cell proliferation assay using NR6R-3T3 mouse fibroblasts. Rizzino, A.et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol.147:120. The ED50 for this effect is typically 25-50 ng/mL in thepresence of 0.1 μg/mL heparin.
Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE: 31 kDa,reducing conditions
Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstituteat 25 μg/mL in sterile PBS that containing at least 0.1% human or bovineserum albumin.
Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.
Gene Name: Fgf8 fibroblast growth factor 8[ Mus musculus ]
Official Symbol: Fgf8
Synonyms: Fgf8;fibroblast growth factor 8; Aigf; Fgf-8; HBGF-8; androgen-induced growth factor;heparin-binding growth factor 8
Gene ID: 14179
mRNA Refseq: NM_001166361
Protein Refseq: NP_001159833
UniProt ID: P37237
Chromosome Location: 19 C3-D; 19 45.0 cM
Pathway: Downstream signalingof activated FGFR; ESC Pluripotency Pathways; FGFR ligand binding andactivation; FGFR2 ligand binding and activation; IRS-mediated signalling;IRS-related events; Insulin receptor signalling cascade; MAPK signalingpathway; Melanoma; PI-3K cascade; Pathways in cancer; PhospholipaseC-mediated cascade; Regulation of Actin Cytoskeleton; DopminergicNeurogenesis; FGFR1 ligand binding and activation
Function: chemoattractantactivity; growth factor activity; type 1 fibroblast growth factor receptorbinding; type 2 fibroblast growth factor receptor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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