Active Recombinant Mouse Chemokine (C-X3-C motif) Ligand 1, His-tagged

• Cat.No.: Cx3cl1-380M
• Product Overview: Recombinantmouse Cx3cl1 protein was expressed in Spodopterafrugiperda, Sf 21 (baculovirus), with a C-terminal 6-His-tagged.Gln25-Arg337.

Specification

• Cat. No.: Cx3cl1-380M
• Description: Cx3cl1 is a largecytokine protein of 373 amino acids; it contains multiple domains and is theonly known member of the CX3C chemokine family. It is alsocommonly known under the names fractalkine (in humans) and neurotactin (in mice).The polypeptide structure of Cx3cl1 differs from thetypical structure of other chemokines.
• Source: Spodopterafrugiperda, Sf 21 (baculovirus)-derived
• Form: Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• N-terminal Sequence: No results obtained:Gln25 predicted
• Molecular Weight: 34 kDa
• Activity: Measured by itsability to chemoattract freshly isolated human peripheral blood lymphocytes(PBL). The ED50 for this effect is typically 0.2-0.6 μg/mL. Measuredby its ability to chemoattract human CX3CR1 transfected BaF3 mouse pro-Bcells. The ED50 for this effect is typically 0.03-0.12 μg/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 10μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Cx3cl1chemokine (C-X3-C motif) ligand 1 [ Mus musculus ]
• Official Symbol: Cx3cl1
• Synonyms: Cx3cl1; chemokine (C-X3-Cmotif) ligand 1; CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747;D8Bwg0439e; fractalkine; neurotactin; C-X3-C motif chemokine 1; small-induciblecytokine D1; CX3C membrane-anchored chemokine; small inducible cytokinesubfamily D, 1; Fkn; Cx3C
• Gene ID: 20312
• mRNA Refseq: NM_009142
• Protein Refseq: NP_033168
• UniProt ID: O35188
• Chromosome Location: 8
• Pathway: Chemokine receptorsbind chemokines; Chemokine signaling pathway; Class A/1 (Rhodesian-likereceptors); Cytokine-cytokine receptor interaction; GPCR ligand binding;Peptide ligand-binding receptors; Signal Transduction; Signaling by GPCR
• Function: chemokine activity; cytokineactivity