Active Recombinant Mouse A Disintegrin-Like And Metallopeptidase (reprolysin type) With Thrombospondin Type 1 Motif, 1, His-tagged

• Cat.No.: Adamts1-441M
• Product Overview: RecombinantMouse Adamts1proteinwas expressed in Chinese Hamster Ovary cell line. Phe254-Cys725,with a C terminal 10 His tag.

Specification

• Cat. No.: Adamts1-441M
• Description: Adamts1 is the foundingmember of the family of secreted zinc proteases with a multi domainstructure. The protein precursor consists of a signal peptide and the followingdomains: pro, catalytic, disintegrinlike, TS type 1 motif, cysteine rich,spacer and a variable number of thrombospondin type 1 Motifs. Based on theirsubstrate specificity, Adamts1 and associated familymembers may be key enzymes in the degradation of cartilage leading toinflammation and arthritis.
• Source: ChineseHamster Ovary cell line, CHO-derived.
• Form: Monomer.Recombinant Mouse Adamts1 is prone to proteolytic cleavage at C terminus.The predominant form of the purified protein lacks the His tag.
• N-terminalSequence: Phe254
• MolecularWeight: 53 kDa
• Activity: Measuredby its ability to cleave rhAggrecan. rm Adamts1 achieves > 50% substrate cleavage, as measured underthe described conditions. See Activity Assay Protocol.
• Endotoxin Level: <1.0EU per 1 ug of the protein by the LAL method.
• Purity: >95%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles.6 months from date of receipt, 20 to 70°C as supplied. 3 months, 20 to 70°Cunder sterile conditions after opening.

Gene Information

• Gene Name: Adamts1 a disintegrin-like andmetallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 [ Musmusculus ]
• Official Symbol: Adamts1
• Synonyms: Adamts1; a disintegrin-likeand metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1; C3-C5; METH1; ADAMTS; METH-1; ADAM-TS1;ADAMTS-1; A disintegrin and metalloproteinase with thrombospondin motifs 1;ADAM-TS 1; a disintegrin-like and metalloprotease (reprolysin type) withthrombospondin type 1 motif, 1
• Gene ID: 11504
• mRNA Refseq: NM_009621
• Protein Refseq: NP_033751
• UniProt ID: P97857
• Chromosome Location: 16
• Pathway: Endochondral Ossification
• Function: heparin binding; hydrolaseactivity; metal ion binding; metalloendopeptidase activity; metallopeptidaseactivity; peptidase activity; protein binding; zinc ion binding