||Recombinant humanCSRP2 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||CSRP2 (Cysteine andglycine-rich protein 2) is a member of the CSRP family, contains 2 LIMzinc-binding domains, which may be involved in regulatory processes importantfor development and cellular differentiation. This protein plays a role inthe development of the embryonic vascular system. CRP2 contains two copies ofthe cysteine-rich amino acid sequence motif (LIM) with putative zinc-bindingactivity, and may be involved in regulating ordered cell growth. Other genesin the family include CSRP1 and CSRP3
||Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 2mM DTT, 200mM NaCl
||23.5kDa (217aa),confirmed by MALDI-TOF
||> 90% by SDS -PAGE
||0.5mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMPVWGG GNKCGACGRT VYHAEEVQCD GRSFHRCCFL CMVCRKNLDS TTVAIHDEEIYCKSCYGKKY GPKGYGYGQG AGTLNMDRGE RLGIKPESVQ PHRPTTNPNT SKFAQKYGGA EKCSRCGDSVYAAEKIIGAG KPWHKNCFRC AKCGKSLEST TLTEKEGEIY CKGCYAKNFG PKGFGYGQGA GALVHAQ
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.