Recombinant Human Eukaryotic Translation Initiation Factor 4H, His-tagged

• Cat.No.: EIF4H-6789H
• Product Overview: Recombinant humanEIF4H protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques

Specification

• Cat. No.: EIF4H-6789H
• Description: Eukaryotictranslation initiation factor 4H, also known as EIF4H, is a 248 amino acidprotein that localizes to the perinuclear region of the cytoplasm and isexpressed as two isoforms, designated short and long. EIF4H induces theRNA-dependent ATP hydrolysis catalyzed by the initiation factors EIF4A andEIF4B. EIF4H was further shown to stimulate the initial rate and extent ofEIF4A-mediated mRNA secondary structure unwinding. Defects in the geneencoding EIF4H are associated with Williams- Beuren syndrome (WBS), a raredevelopmental disorder characterized by cardiovascular and musculo-skeletalabnormalities
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
• Molecular Weight: 29.9 kDa (272aa),confirmed by MALDI-TOF
• Purity: > 90% by SDS -PAGE
• Concentration: 0.5 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMADFDT YDDRAYSSFG GGRGSRGSAG GHGSRSQKEL PTEPPYTAYV GNLPFNTVQGDIDAIFKDLSIRSVRLVRDK DTDKFKGFCY VEFDEVDSLK EALTYDGALL GDRSLRVDIA EGRKQDKGGFGFRKGGPDDR GMGSSRESRG GWDSRDDFNS GFRDDFLGGR GGSRPGDRRT GPPMGSRFRD GPPLRGSNMDFREPTEEERA QRPRLQLKPR TVATPLNQVA NPNSAIFGGA RPREEVVQKEQE
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: EIF4Heukaryotic translation initiation factor 4H [ Homo sapiens ]
• Official Symbol: EIF4H
• Synonyms: EIF4H; eukaryotic translationinitiation factor 4H; WBSCR1, Williams Beuren syndrome chromosome region 1;KIAA0038; WSCR1; E430026L18Rik; Ef4h; AU018978; D5Ertd355e; eIF 4H; eIF-4H;EIF4H; Eukaryotic translation initiation factor 4H; IF4H_HUMAN; KIAA0038;WBSCR1; Williams Beuren syndrome chromosomal region 1 protein homolog;Williams Beuren syndrome chromosome region 1; Williams-Beuren syndromechromosomal region 1 protein; WSCR1; Williams-Beuren syndrome chromosomeregion 1; WBSCR1; eIF-4H
• Gene ID: 7458
• mRNA Refseq: NM_031992
• Protein Refseq: NP_114381
• MIM: 603431
• Uniprot ID: Q15056
• Chromosome Location: 7q11.23
• Pathway: Activation of the mRNA upon bindingof the cap-binding complex and eIFs, and subsequent binding to 43S,organism-specific biosystem; Cap-dependent Translation Initiation,organism-specific biosystem; Eukaryotic Translation Initiation,organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomalsubunit, organism-specific biosystem; Gene Expression, organism-specificbiosystem
• Function: RNA binding; nucleotide binding;protein binding; translation factor activity, nucleic acid binding;translation initiation factor activity