||Recombinant human CLIC2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography(1-247aa).
||CLIC2, also known as, chloride intracellular channel protein 2, regulates cellular traffic of chloride ions, a critical component of all living cells. This protein is involved in membrane potential stabilization, signal transduction, cell volume regulation and organic solute transport. It is detected in fetal liver and adult skeletal muscle tissue. It is a potential candidate for one of the many diseases linked to Xq28.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl and 1mM DTT
||30.5 kDa(267aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSGLRPGTQV DPEIELFVKA GSDGESIGNC PFCQRLFMIL WLKGVKFNVT TVDMTRKPEE LKDLAPGTNP PFLVYNKELK TDFIKIEEFL EQTLAPPRYP HLSPKYKESF DVGCNLFAKF SAYIKNTQKE ANKNFEKSLL KEFKRLDDYL NTPLLDEIDP DSAEEPPVSR RLFLDGDQLT LADCSLLPKL NIIKVAAKKY RDFDIPAEFS GVWRYLHNAY AREEFTHTCP EDKEIENTYA NVAKQKS
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.