||Recombinanthuman HDAC6 was expressed in E. coli as the full length protein with anN-terminal FLAG tag. The molecular weight of the protein is 132 kDa.
||HDAC6is a histone deacetylase containig two HDAC catalytic domains. HDAC6 is anenzyme that in humans is encoded by the HDAC6 gene. Histones play a critical rolein transcriptional regulation, cell cycle progression, and developmentalevents. It contains an internal duplication of two catalytic domains whichappear to function independently of each other.
||20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20%glycerol.
||10g recombinant HDAC6 protein, 0.5 g/l.
|Dilution of Protein:
||RecombinantHDAC6 is supplied in solution and should be kept as a solution in ordertomaintain full activity.
||RecombinantHDAC6 is suitable for use in histone deacetylase assays. Arecommendedstarting point for the HDAC assay is 20-100 ng of recombinant proteinperreaction.
||Recombinantproteins in solution are temperature sensitive and should be stored at-80°Cto prevent degradation. Avoid repeated freeze/thaw cycles and keep on icewhennot in storage.