Active Recombinant Human Histone Deacetylase 6, His-tagged

• Cat.No.: HDAC6-401H
• Product Overview: Recombinanthuman HDAC6 was expressed in insect cells with Histag. The molecular weight of the protein is 131 kDa.

Specification

• Cat. No.: HDAC6-401H
• Description: HDAC6is a class II HDAC, a group defined by its member's homology to the yeastHDAC HDA1. It is unique among human HDACs in that it contains two fulldeacetylase domains. A tubulin deacetylase, HDAC6 may act to promoteautophagic clearing of huntingtin aggregates and retard HIV1 infection, butalso to promote invasive motility in tumor cells.
• Source: Producedin insect cells.
• Species: Human
• Supplied as: XX μg/μl in 50 mM Tris,pH 8.0, 138 mM NaCl and 10% glycerol.
• Purity: > 90% bySDS-PAGE.
• Molecular Weight: 131kDa (1215 aa)
• Activity: > 2 U/μg.One U=1 pmol/min at 37°C, 100 μM. Active with various fluorogenic peptidesubstrates, including Fluor de Lys™ substrate and Fluor de Lys™-SIRT1.
• Application: Study HDAC6kinetics and inhibitor sensitivity.
• Long Term Storage: -80°C.
• Storage: -70°C.The enzyme is stable on ice for the time typically required to set up anexperiment (30-60 min.), but may lose activity with prolonged storage on ice.It is recommended that thawing and dilution of the enzyme be done within asshort a time as possible before start of the assay. The remaining, unused,undiluted enzyme should be refrozen quickly by, for example, snap freezing ina dry/ice ethanol bath or liquid nitrogen. Freezing and storage of dilutedenzyme is not recommended.
• Publication:
  Circumvention of Gefitinib Resistance by Repurposing Flunarizine via Histone Deacetylase Inhibition (2023)

Gene Information

• Gene Name: HDAC6 histonedeacetylase 6 [ Homo sapiens ]
• Official Symbol: HDAC6
• Synonyms: HDAC6;histone deacetylase 6; HD6; JM21; FLJ16239; EC 3.5.1.98; KIAA0901;OTTHUMP00000032398; OTTHUMP00000197663
• Gene ID: 10013
• mRNA Refseq: NM_006044
• Protein Refseq: NP_006035
• MIM: 300272
• UniProt ID: Q9UBN7
• Chromosome Location: Xp11.23
• Pathway: Cell cycle; NOTCH1 Intracellular DomainRegulates Transcription; Signal Transduction; Signaling by NOTCH; Signalingby NOTCH1; Signaling events mediated by HDAC Class I; Signaling eventsmediated by HDAC Class II; TNF-alpha/NF-kB Signaling Pathway
• Function: Hsp90 protein binding; NAD-dependenthistone deacetylase activity (H3-K14 specific); NAD-dependent histonedeacetylase activity (H3-K9 specific); NAD-dependent histone deacetylaseactivity (H4-K16 specific); actin binding; alpha-tubulin binding;beta-catenin binding; beta-tubulin binding; dynein complex binding; enzymebinding; histone deacetylase activity; histone deacetylase activity (H3-K16specific); histone deacetylase binding; hydrolase activity; metal ion binding