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Recombinant Human Histidine Ammonia-Lyase

Cat. No.: HAL-2513H
Product Overview: Recombinant Human Histidine Ammonia-Lyase protein, was expressed in Human HEK293 cells and purified by SDS-PAGE and Coomassie blue staining
Description: Histidine ammonia-lyase (or histidase, or histidinase) is an enzyme that in humans is encoded by the HAL gene. Histidase converts histidine into ammonia and urocanic acid. Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid.
Source: Human HEK293 cells
Preparation: HEK293T cells were transiently transfected with MegaTran Transfection Reagent and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit.
Molecular Weight: 72.5 kDa
Purity: > 80% as determinedby SDS-PAGE and Coomassie blue staining
Concentration: > 50 ug/ml as determined by BCA
Buffer: 10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3
Storage: Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions
Gene Name: HAL, histidine ammonia-lyase [Homo sapiens]
Official Symbol: HAL
Synonyms: HAL; histidine ammonia-lyase; HIS; HSTD; histidine ammonia-lyase; histidase
Gene ID: 3034
mRNA Refseq: NM_002108
Protein Refseq: NP_002099
MIM: 609457
UniProt ID: P42235
Chromosome Location: 12q22-q24.1
Pathway: Histidine catabolism; Histidine metabolism; Metabolic pathways; Metabolism of amino acids and derivatives; Nitrogen metabolism; histidine degradation III; histidine degradation VI
Function: histidine ammonia-lyase activity; lyase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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