||RecombinantHuman ERBB2 protein, was expressed in Human HEK293 cells and purified bySDS-PAGE and Coomassie blue staining
||This gene encodes amember of the epidermal growth factor receptor (EGFR) family of receptortyrosine kinases. This membrane-bound protein has a neuregulin binding domainbut not an active kinase domain. It therefore can bind this ligand but notconvey the signal into the cell through protein phosphorylation. However, itdoes form heterodimers with other EGF receptor family members which do havekinase activity. Heterodimerization leads to the activation of pathways whichlead to cell proliferation or differentiation. Amplification of this gene and/oroverexpression of its protein have been reported in numerous cancers,including prostate, bladder, and breast tumors. Alternate transcriptionalsplice variants encoding different isoforms have been characterized. Oneisoform lacks the intermembrane region and is secreted outside the cell. Thisform acts to modulate the activity of the membrane-bound form. Additionalsplice variants have also been reported, but they have not been thoroughlycharacterized.
||Human HEK293 cells
||HEK293Tcells were transiently transfected with MegaTran Transfection Reagent and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrsbefore collection. The cells were lysed in modified RIPA buffer (25mMTris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitorcocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify thelysate. Recombinant proteins were purified through anti-DDK affinity columns.Protein concentration was measured by BCA kit.
||> 80%as determined by SDS-PAGE and Coomassie blue staining
||> 50ug/ml as determined by BCA
||10%glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3
||Store at-80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt ofproducts under proper storage and handling conditions