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Recombinant Human ERBB2

Cat. No.: ERBB2-2515H
Product Overview: RecombinantHuman ERBB2 protein, was expressed in Human HEK293 cells and purified bySDS-PAGE and Coomassie blue staining
Description: This gene encodes amember of the epidermal growth factor receptor (EGFR) family of receptortyrosine kinases. This membrane-bound protein has a neuregulin binding domainbut not an active kinase domain. It therefore can bind this ligand but notconvey the signal into the cell through protein phosphorylation. However, itdoes form heterodimers with other EGF receptor family members which do havekinase activity. Heterodimerization leads to the activation of pathways whichlead to cell proliferation or differentiation. Amplification of this gene and/oroverexpression of its protein have been reported in numerous cancers,including prostate, bladder, and breast tumors. Alternate transcriptionalsplice variants encoding different isoforms have been characterized. Oneisoform lacks the intermembrane region and is secreted outside the cell. Thisform acts to modulate the activity of the membrane-bound form. Additionalsplice variants have also been reported, but they have not been thoroughlycharacterized.
Host: Human HEK293 cells
Preparation: HEK293Tcells were transiently transfected with MegaTran Transfection Reagent and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrsbefore collection. The cells were lysed in modified RIPA buffer (25mMTris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitorcocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify thelysate. Recombinant proteins were purified through anti-DDK affinity columns.Protein concentration was measured by BCA kit.
Molecular Weight: 137.7 kDa
Purity: > 80%as determined by SDS-PAGE and Coomassie blue staining
Concentration: > 50ug/ml as determined by BCA
Buffer: 10%glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3
Storage: Store at-80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt ofproducts under proper storage and handling conditions
Gene Name: ERBB2v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian) [ Homo sapiens ]
Official Symbol: ERBB2
Synonyms: ERBB2; v-erb-b2 erythroblasticleukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogenehomolog (avian); NEU; NGL; HER2; TKR1; CD340; HER-2; HER-2/neu; Receptortyrosine-protein kinase erbB-2; p185erbB2; C-erbB-2; Tyrosine kinase-typecell surface receptor HER2; MLN 19; c-erb B2/neu prote;neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblasticleukemia viral oncogene; homolog 2 (neuro/glioblastoma derived oncogenehomolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2
Gene ID: 2064
mRNA Refseq: NM_004448
Protein Refseq: NP_004439
MIM: 164870
UniProt ID: P04626
Chromosome Location: 17q11.2-q12
Pathway: Adherens junction; Bladder cancer;Calcium signaling pathway; Endometrial cancer; ErbB signaling pathway; Focaladhesion; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer;Prostate cancer; Axon guidance
Function: ATP binding; ErbB-3 class receptorbinding; Hsp90 protein binding; epidermal growth factor receptor activity;glycoprotein binding; growth factor binding; identical protein binding;nucleotide binding; protein C-terminus binding; protein heterodimerizationactivity; protein phosphatase binding; protein phosphatase binding; receptorsignaling protein tyrosine kinase activity; transferase activity;transmembrane receptor activity; ubiquitin protein ligase bindin

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