||Theprotein was expressed as gst-tagged fusion protein by E.Coli and puried by GSH-sepharose. The puried protein was resolved in 1M PBS (58mM Na2HP O4,17mMNaH2P O4, 68mM NaCl, pH8. ) added with 100mM GS H and 1% Triton X-100,15%glycerol
||Zinc finger proteinZPR1 is a protein that in humans is encoded by the ZNF259 gene
||58mM Na2HPO4,17mM NaH2P O4, 68mM NaCl, pH8.
||1M PBS(58mM Na2HP O4,17mM NaH2P O4, 68mM NaCl, pH8. ) added with 100mM GS H and 1%Triton X- 100,15%glycerol
||Store inPBS buffer at -20 oC. Repeated freeze thaw cycles should beavoided