||Bovine Myelinbasic protein, was highly Purified from bovinecentral nervous system tissue by method of Määttä et al.
||MBP is a peripheralmembrane protein, and its interaction with lipids is generally believed to becritical for the formation and stability of the multilamellar myelin sheath.It has been identified as a Ca2+-calmodulin regulated agent foractin polymerization, tropomyosin and actomyosin function, tubulinstabilization, and clathrin assembley. MBP is generally considered to be theantigen responsible for autoimmunity in multiple sclerosis (MS) and it hasthe capability to induce experimental allergic encephalomyelitis (EAE). MBPis also an endogenous inhibitor of the high-affinity cannabinoid binding sitein the brain and a substrate for numerous protein kinases (MAPK,serine/threonine kinase, protein kinase C). MBP is also suggested to beinvolved in intracellular signalling and as cytoskeletal components.
||bovine central nervous system tissue
||≥95% (SDS-PAGE; immunoblot)
||Lyophilized. Essentially salt-free.
||Reconstitute with distilled water.
||Substratefor various kinases including MAPK, PKA, calmodulin-dependent protein kinase,PKC and phosphorylase kinase. Raf1, MEK, and MEKK can also use MBP as asubstrate.
||Stablefor at least 1 year when stored at +4°C. Reconstituted protein is stable forat least 6 months at -20°C and for at least 24 hours at +37°C in aqueoussolution.