||Recombinant human HSPB11 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-144aa).
||HSPB11, also known as PP25, is detected in placenta.
||Liquid. In 20mM Tris-HClbuffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
||18.5 kDa (164aa), confirmed by MALDI-TOF(Molecular weight on SDS-PAGE will appear higher)
||>90 % by SDS -PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MRKIDLCLSS EGSEVILATS SDEKHPPENI IDGNPETFWT TTGMFPQEFI ICFHKHVRIE RLVIQSYFVQ TLKIEKSTSK EPVDFEQWIE KDLVHTEGQL QNEEIVAHDG SATYLRFIIV SAFDHFASVH SVSAEGTVVS NLSS
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.