||Recombinant human SNRPB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-225aa).
||SNRPB2, also known as U2 small nuclear ribonucleoprotein B, belongs to the RRM U1 A/B family. This protein associates with stem loop IV of U2 small nuclear ribonucleoprotein (U2 snRNP) in the presence of snRNP-A. It may play a role in pre-mRNA splicing. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding the same protein have been found for this gene.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl
||27.6 kDa (245aa), confirmed by MALDI-TOF
||>90% by SDS - PAGE
||0.25 mg/ml (determined by Bradfordassay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSDI ISKMRGTFAD KEKKKEKKKA KTVEQTATTT NKKPGQGTPN SANTQGNSTP NPQVPDYPPN YILFLNNLPE ETNEMMLSML FNQFPGFKEV RLVPGRHDIA FVEFENDGQA GAARDALQGF KITPSHAMKITYAKK
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.