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Rabbit Anti-Human Dickkopf 1 Homolog Monoclonal Antibody

Cat.No. : CAB11524RH
Product Overview : Rabbit Monoclonal Antibody to Human Dickkopf 1 Homolog
  • Specification
  • Gene Information
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Antigen Description : DKK1 ( dickkopf related protein 1 ) is a head inducer secreted from the vertebrate head organizer and belongs to the DKK protein family including four members. DKK1 is synthesized initially as a precursor protein with an N-terminal signal peptide and two cysteine-rich domains. DKK proteins have distinct patterns of expression in adult and embryonic tissues and have effects on Wnt signaling implicated in diverse processes. Dkk1 is a specific and high-affinity ligand for LRP6 ( LDL-receptor-related protein 6 ) acting as a Wnt co-receptor. Dkk1 blocks LRP6-mediated Wnt/beta-catenin signaling by antagonistically inhibiting the formation of Wnt-Fz-LRP6 complex which triggers the Wnt signal pathway. It is revealed that Wnt signaling in osteoblasts is necessary to promote BMP-2-mediated differentiation of pre-osteoblast cells, and therefore, DKK1 may play a key role in regulating pre-osteoblast differentiation and the development of multiple myeloma-associated osteolytic bone lesions.
Specificity : Human Dkk1 / Dickkopf-1. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen : Recombinant human Dkk1 protein
Host : Rabbit
Isotype : Rabbit IgG
Species : Human
Clone : A005
Applications : WB; ELISA
Dilution : Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human Dkk1 in WB. Using a DAB detection system, the detection limit for Dkk1 is approximately 1 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommendedELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human Dkk1. The detection limit for human Dkk1 is approximately 0.00975 ng/well
Preparation : This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human dickkopf related protein 1.
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
Official Symbol : DKK1
Synonyms : DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1;
Gene ID : 22943
mRNA Refseq : NM_012242
Protein Refseq : NP_036374
MIM : 605189
UniProt ID : O94907
Chromosome Location : 10q11.2
Pathway : Direct p53 effectors, organism-specific biosystem; Presenilin action in Notch and Wnt signaling, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem; Wnt Signaling Pathway NetPath, organism-specific biosystem; Wnt signaling network, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem;
Function : growth factor activity; low-density lipoprotein particle receptor binding; protein binding; receptor antagonist activity; signal transducer activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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