Recombinant Human APEX1 protein(Pro2-Leu318), His-tagged

• Cat.No.: APEX1-2497H
• Product Overview: Recombinant Human APEX1 (P27695) (Pro2-Leu 318) was expressed in E. coli, with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: Pro2-Leu318
• Form: Lyophilized from sterile PBS, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human APEX1 consisting of 328 amino acids and migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions as predicted.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1 [ Homo sapiens ]
• Official Symbol: APEX1
• Synonyms: APEX1; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX, APEX nuclease (multifunctional DNA repair enzyme); DNA-(apurinic or apyrimidinic site) lyase; APE; APE 1; APEN; APX; HAP1; REF 1; REF1; AP lyase; protein REF-1; redox factor-1; AP endonuclease class I; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); APE1; APEX
• Gene ID: 328
• mRNA Refseq: NM_001244249
• Protein Refseq: NP_001231178
• MIM: 107748
• UniProt ID: P27695