Active Recombinant Mouse Hmgb1 protein(Met1-Glu215), hFc-tagged

• Cat.No.: Hmgb1-6948M
• Product Overview: Recombinant Mouse HMGB1 (P63158) (Met 1-Glu 215) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N-hFc
• Protein length: Met1-Glu215
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind mouse AGER-His in functional ELISA.
• Molecular Mass: The recombinant mouse HMGB1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 475 amino acids and has a calculated molecular mass of 53.3 kDa. The apparent molecular mass of the monomer is approximately 57 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Hmgb1 high mobility group box 1 [ Mus musculus ]
• Official Symbol: Hmgb1
• Synonyms: HMGB1; high mobility group box 1; high mobility group protein B1; high mobility group protein 1; DEF; p30; Hmg1; HMG-1; SBP-1; amphoterin; MGC103168; MGC103169; MGC117896; MGC117897
• Gene ID: 15289
• mRNA Refseq: NM_010439
• Protein Refseq: NP_034569