Active Recombinant Human NRP1 Protein(Met 1-Lys 644, with natural variants V179A), His-tagged

• Cat.No.: NRP1-090H
• Product Overview: Active Recombinant Human NRP1 Protein(NP_001019799.2, with natural variants V179A) (Met 1-Lys 644) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Using the Octet RED System, the affinity constant (Kd) of NRP1-his bound to biotinylated human VEGF165 was 25nM.
  2. Immobilized Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human Neuropilin-1 / NRP1 / CD304 Protein (His Tag) with a linear range of 32-160 μg/mL.
• Molecular Mass: The secreted recombinant human NRP1 consists of 634 amino acids and has a calculated molecular mass of 71.3 kDa. As a result of glycosylation, rh NRP1 migrates as an approximately 88 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Met 1-Lys 644, with natural variants V179A
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRP1 neuropilin 1 [ Homo sapiens ]
• Official Symbol: NRP1
• Synonyms: NRP1; neuropilin 1; neuropilin-1; CD304; NRP; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; NP1; BDCA4; DKFZp781F1414; DKFZp686A03134
• Gene ID: 8829
• mRNA Refseq: NM_001024628
• Protein Refseq: NP_001019799
• MIM: 602069
• UniProt ID: O14786