Active Recombinant Rat Vegfa protein(Met1-Arg190)

• Cat.No.: Vegfa-878R
• Product Overview: Recombinant Rat VEGF 164 isoform (AAA41211.1) (Met 1-Arg 190) was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Rat
• Tag: N/A
• Protein length: Met1-Arg190
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-10 ng/ml.
• Molecular Mass: The secreted recombinant rat VEGF164 consists of 164 amino acids and has a predicted molecular mass of 19.2 kDa. The apparent molecular mass of the rat VEGF164 is approximately 25 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 86 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Rattus norvegicus ]
• Official Symbol: Vegfa
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vegf; VEGF164
• Gene ID: 83785
• mRNA Refseq: NM_001110333
• Protein Refseq: NP_001103803