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Recombinant Human Carbonic Anhydrase I, His-tagged

Cat.No.: CA1-2505H
Product Overview: Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 31.0 kDa.
Description: CA1 is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O<->HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
Molecular Mass: 31.0 kDa (281aa), confirmed by MALDI-TOF
Protein length: 1-261aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGS LT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
Purity: >95% by SDS-PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: CA1 carbonic anhydrase I [ Homo sapiens ]
Official Symbol: CA1
Synonyms: CA1; carbonic anhydrase I; carbonic anhydrase 1; Car1; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase I; CAB; CA-I;
Gene ID: 759
mRNA Refseq: NM_001128829
Protein Refseq: NP_001122301
MIM: 114800
UniProt ID: P00915
Chromosome Location: 8q21.2
Pathway: C-MYB transcription factor network, organism-specific biosystem;
Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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