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Recombinant Human Carbonic Anhydrase I, His-tagged

Cat.No. : CA1-2505H
Product Overview : Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 31.0 kDa.
Description : CA1 is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O<->HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
Molecular Mass : 31.0 kDa (281aa), confirmed by MALDI-TOF
Protein length : 1-261aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGS LT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
Purity : >95% by SDS-PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : CA1 carbonic anhydrase I [ Homo sapiens ]
Official Symbol : CA1
Synonyms : CA1; carbonic anhydrase I; carbonic anhydrase 1; Car1; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase I; CAB; CA-I;
Gene ID : 759
mRNA Refseq : NM_001128829
Protein Refseq : NP_001122301
MIM : 114800
UniProt ID : P00915
Chromosome Location : 8q21.2
Pathway : C-MYB transcription factor network, organism-specific biosystem;
Function : carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding;

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