Recombinant Human ADP-Ribosylation Factor-Like 3, His-tagged

• Cat.No.: ARL3-6872H
• Product Overview: Recombinant human ARL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 23.0 kDa.

Specification

• Description: ARL3 is a member of the ARF-like protein (ARL) subfamily of small GTPases. Unlike ARFs, ARL3 does not activate the cholera toxin ADP-ribosyltranferase. This protein is ubiquitously expressed. It may play a role in maintaining rod photoreceptor cells and regulating microtubule-dependent processes. Knockdown of ARL3 leads to altered cell morphology, failure of cytokinesis, acetylation of alpha tubulin and an increase in binucleated cells.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol 0.1M NaCl,1mM DTT, 0.1mM PMSF.
• Molecular Mass: 23.0 kDa (206aa), confirmed by MALDI-TOF.
• Protein length: 1-182aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGLLSI LRKLKSAPDQ EVRILLLGLD NAGKTTLLKQ LASEDISHIT PTQGFNIKSV QSQGFKLNVW DIGGQRKIRP YWKNYFENTD ILIYVIDSAD RKRFEETGQE LAELLEEEKL SCVPVLIFAN KQDLLTAAPA SEIAEGLNLH TIRDRVWQIQ SCSALTGEGV QDGMNWVCKN VNAKKK
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ARL3 ADP-ribosylation factor-like 3 [ Homo sapiens ]
• Official Symbol: ARL3
• Synonyms: ARL3; ADP-ribosylation factor-like 3; ADP-ribosylation factor-like protein 3; ARFL3; ARF-like 3
• Gene ID: 403
• mRNA Refseq: NM_004311
• Protein Refseq: NP_004302
• MIM: 604695
• UniProt ID: P36405
• Chromosome Location: 10q23.3
• Function: GDP binding; GTP binding; GTPase activity; metal ion binding; microtubule binding; nucleotide binding; protein binding