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Recombinant Human Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha, His-tagged

Cat.No. : NFKBIA-6897H
Product Overview : Recombinant human NFKBIA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 37.7 kDa.
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Description : NFKBIA is members of the IkB family of proteins can be divided into four groups (IkB-alpha, IkB-beta, IkB-gamma, IkB-epsilon). NFKBIA inhibits the NFkB complex by binding and sequestering it in the cytoplasm. Upon stimulation, NFKBIA is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl.
Molecular Mass : 37.7 kDa (337aa), confirmed by MALDI-TOF
Protein length : 1-317aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MFQAAERPQE WA MEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN YNGHTCLHLA SI HGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE DELPYDDCVF GGQRLTL
Purity : >90% by SDS-PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/ml (determined by Bradford assay)
Gene Name : NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha [ Homo sapiens ]
Official Symbol : NFKBIA
Synonyms : NFKBIA; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; NFKBI; NF-kappa-B inhibitor alpha; IkappaBalpha; IKBA; MAD 3; ikB-alpha; I-kappa-B-alpha; nuclear factor of kappa light chain gene enhancer in B-cells; major histocompatibility complex enhancer-binding protein MAD3; MAD-3;
Gene ID : 4792
mRNA Refseq : NM_020529
Protein Refseq : NP_065390
MIM : 164008
UniProt ID : P25963
Chromosome Location : 14q13
Pathway : Activated TLR4 signalling, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem;
Function : NF-kappaB binding; NF-kappaB binding; enzyme binding; heat shock protein binding; identical protein binding; nuclear localization sequence binding; protein binding; transcription factor binding; ubiquitin protein ligase binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the interaction between NFKBIA protein and NF-κB? 11/26/2022

The interaction between NFKBIA protein and NF-κB can be identified by co-immunoprecipitation, bimolecular fluorescence complementation, etc. In addition, these methods can also be used to study the interaction sites and mechanisms of action between them.

What is the effect of knocking out the NFKBIA gene on cell growth and apoptosis? 11/21/2022

Knockdown of the NFKBIA gene leads to increased cell growth arrest and apoptosis, which may be related to the aberrant activation of the NF-κB signaling pathway.

How can I study the effect of NFKBIA protein on the NF-κB signaling pathway? 04/17/2022

The effect on the expression level and activity of molecules related to the NF-κB signaling pathway can be observed by overexpressing or knocking down NFKBIA protein. In addition, studies can also be performed with specific knockout or mutant cell lines.

Is it possible to knock out NFKBIA by gene knockout? 01/08/2022

Its role in physiological and pathological processes can be studied by specifically knocking out the NFKBIA gene using specific short hairpin RNA or zinc finger nuclease technology.

How does the expression level of NFKBIA protein relate to disease? 07/07/2020

Studies have shown that the expression level of NFKBIA protein is associated with the occurrence and progression of a variety of diseases. For example, in some cancers, the expression levels of the NFKBIA protein are reduced or missing, leading to aberrant activation of the NF-κB signaling pathway.

Do NFKBIA proteins regulate the NF-κB signaling pathway? 01/21/2020

When cells are stimulated by inflammation, the NFKBIA protein is phosphorylated, causing its dissociation from NF-κB. After dissociation, NF-κB is transferred to the nucleus and activates the transcription of specific genes. In addition, NFKBIA protein can further regulate the activity of NF-κB by interacting with other proteins.

Customer Reviews (3)

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Reviews
07/04/2022

    I am very happy with the results, they performed very well in my experiments.

    05/26/2022

      It has high sensitivity and strong specificity.

      04/07/2022

        It is easy to use and requires little preparation before the experiment.

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