Recombinant Human Cyclin B2, His-tagged

• Cat.No.: CCNB2-6835H
• Product Overview: Recombinant human CCNB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Description: CCNB2 belongs to the cyclin family. CCNB2 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT.
• Molecular Mass: 47.9 kDa (422aa) confirmed by MALDI-TOF
• Protein length: 1-398aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMALLRR PTVSSDLENI DTGVNSKVKS HVTIRRTVLE EIGNRVTTRA AQVAKKAQNT KVPVQPTKTT NVNKQLKPTA SVKPVQMEKL APKGPSPTPE DVSMKEENLC QAFSDALLCK IEDIDNEDWE NPQLCSDYVK DIYQYLRQLE VLQSINPHFL DGRDINGRMR AILVDWLVQV HSKFRLLQET LYMCVGIMDR FLQVQPVSRK KLQLVGITAL LLASKYEEMF SPNIEDFVYI TDNAYTSSQI REMETLILKE LKFELGRPLP LHFLRRASKA GEVDVEQHTL AKYLMELTLI DYDMVHYHPS KVAAAASCLS QKVLGQGKWN LKQQYYTGYT ENEVLEVMQH MAKNVVKVNE NLTKFIAIKN KYASSKLLKI SMIPQLNSKA VKDLASPLIG RS
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CCNB2 cyclin B2 [ Homo sapiens ]
• Official Symbol: CCNB2
• Synonyms: CCNB2; cyclin B2; G2/mitotic-specific cyclin-B2; HsT17299
• Gene ID: 9133
• mRNA Refseq: NM_004701
• Protein Refseq: NP_004692
• MIM: 602755
• UniProt ID: O95067
• Chromosome Location: 15q21.3
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem
• Function: protein kinase binding