Recombinant Human Egl Nine Homolog 3 (C. elegans), His-tagged

• Cat.No.: EGLN3-6851H
• Product Overview: Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Description: EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the a subunit of hypoxia-inducible factor-a, which targets hypoxia-inducible factor-a for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau tumor suppressor.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTA.
• Molecular Mass: 29.8 kDa (263aa) confirmed by MALDI-TOF
• Protein length: 1-239aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMPLGHI MRLDLEKIAL EYIVPCLHEV GFCYLDNFLG EVVGDCVLER VKQLHCTGAL RDGQLAGPRA GVSKRHLRGD QITWIGGNEE GCEAISFLLS LIDRLVLYCG SRLGKYYVKE RSKAMVACYP GNGTGYVRHV DNPNGDGRCI TCIYYLNKNW DAKLHGGILR IFPEGKSFIA DVEPIFDRLL FFWSDRRNPH EVQPSYATRY AMTVWYFDAE ERAEAKKKFR NLTRKTESAL TED
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -71°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: EGLN3 egl nine homolog 3 (C. elegans) [ Homo sapiens ]
• Official Symbol: EGLN3
• Synonyms: EGLN3; egl nine homolog 3 (C. elegans); EGL nine (C.elegans) homolog 3; egl nine homolog 3; HIF prolyl hydroxylase 3; HIFPH3; PHD3; HPH-1; HPH-3; HIF-PH3; HIF-prolyl hydroxylase 3; egl nine-like protein 3 isoform; hypoxia-inducible factor prolyl hydroxylase 3; prolyl hydroxylase domain-containing protein 3; FLJ21620; MGC125998; MGC125999
• Gene ID: 112399
• mRNA Refseq: NM_022073
• Protein Refseq: NP_071356
• MIM: 606426
• UniProt ID: Q9H6Z9
• Chromosome Location: 14q12
• Pathway: HIF-1-alpha transcription factor network, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Pathways in cancer, organism-specific biosystem; Renal cell carcinoma, organism-specific biosystem; Renal cell carcinoma, conserved biosystem
• Function: L-ascorbic acid binding; iron ion binding; metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding