Recombinant Human Inositol-Tetrakisphosphate 1-kinase, His-tagged

• Cat.No.: ITPK1-1351H
• Product Overview: Recombinant human ITPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Inositol-tetrakisphosphate 1-kinase, also known as ITPK1, is a 414 amino acid monomer that belongs to the ITPK1 family and exists as two alternatively spliced isoforms. ITPK1 is found at highest levels in brain, followed by heart, skeletal muscle, kidney, pancreas, liver, placenta and lung. ITPK1 contains one ATP-grasp domain and has been found to phosphorylate various inositol polyphosphates and modify TNFalpha induced apoptosis.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA.
• Molecular Mass: 48.1 kDa (438aa), confirmed by MALDI-TOF
• Protein length: 1-414aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMQTFLK GKRVGYWLSE KKIKKLNFQA FAELCRKRGM EVVQLNLSRP IEEQGPLDVI IHKLTDVILE ADQNDSQSLE LVHRFQEYID AHPETIVLDP LPAIRTLLDR SKSYELIRKI EAYMEDDRIC SPPFMELTSL CGDDTMRLLE KNGLTFPFIC KTRVAHGTNS HEMAIVFNQE GLNAIQPPCV VQNFINHNAV LYKVFVVGES YTVVQRPSLK NFSAGTSDRE SIFFNSHNVS KPESSSVLTE LDKIEGVFER PSDEVIRELS RALRQALGVS LFGIDIIINN QTGQHAVIDI NAFPGYEGVS EFFTDLLNHI ATVLQGQSTA MAATGDVALL RHSKLLAEPA GGLVGERTCS ASPGCCGSMM GQDAPWKAEA DAGGTAKLPH QRLGCNAGVS PSFQQHCVAS LATKASSQ
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ITPK1 inositol-tetrakisphosphate 1-kinase [ Homo sapiens ]
• Official Symbol: ITPK1
• Synonyms: ITPK1; inositol-tetrakisphosphate 1-kinase; inositol 1,3,4 triphosphate 5/6 kinase; ins(1,3,4)P(3) 5/6-kinase; inositol-triphosphate 5/6-kinase; inositol 1,3,4-triphosphate 5/6 kinase; inositol 1,3,4-trisphosphate 5/6 kinase; inositol 1,3,4-trisphosphate 5/6-kinase; ITRPK1
• Gene ID: 3705
• mRNA Refseq: NM_001142593
• Protein Refseq: NP_001136065
• MIM: 601838
• UniProt ID: Q13572
• Chromosome Location: 14q32.12
• Pathway: 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian), organism-specific biosystem; 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian), conserved biosystem; 1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3), organism-specific biosystem; D-myo-inositol (1,4,5,6)-tetrakisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,4,5,6)-tetrakisphosphate biosynthesis, conserved biosystem; D-myo-inositol (3,4,5,6)-tetrakisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (3,4,5,6)-tetrakisphosphate biosynthesis, conserved biosystem
• Function: ATP binding; catalytic activity; hydrolase activity; inositol tetrakisphosphate 1-kinase activity; inositol-1,3,4-trisphosphate 5-kinase activity; inositol-1,3,4-trisphosphate 6-kinase activity; isomerase activity; kinase activity; ligase activity; magnesium ion binding; nucleotide binding; transferase activity